Lab 11: Protein Electrophoresis Analysis

On Saturday we did a variation of Wednesday's lab, which has terrible results. The variation is was as follows:

1. Get into four groups

2. Each group will do a different sample.

2a. Bean

2b. Quinoa

2c. Liver

2d. Fish

3. Using the above sample given, follow the grid below

Label 6 micro tubes as follows

Well 1. (5% vinegar): add 20ul of Sample, add 15 ul of 5% vinegar, add 12 ul of loading dye, incubate at room temp for 5 min, add 20ul to gel

Well 2 (5% NaCl): add 20ul of Sample, add 15ul of 5% NaCl, add 12 ul of loading dye, incubate at room temp for 5 min, add 20ul to gel

Well 3 (35% NaCl): add 20ul of Sample, add 15ul of 35% NaCl, add 12 ul of loading dye, incubate at room temp for 5 min, add 20ul to gel

Well 4 (95 C): add 20ul of Sample, add 15ul of water, add 12 ul of loading dye, incubate at 95 C for 5 min, add 20ul to gel

Well 5 (37 C): add 20ul of Sample, add 15ul of water, add 12 ul of loading dye, incubate at 37 C for 5 min, add 20ul to gel

Well 6 (Normal): add 20ul of Sample, add 15ul of water, add 12 ul of loading dye, incubate at room temp for 5 min, add 20ul to gel,

Well 7 (Negative control) Loading Dye , add 20ul to gel

4. Once gels are loaded, run at 130 V for 1 hour.

5. Upon completion of gels, stain for 1 hour

6. Remove stain and allow to sit over night in distilled water

This protocol actually yielded results and so today all classes will be analyzing the results. Since A and D blocks actually did this lab, they will provide feedback on how to improve the lab for next year's students.